Titlebook: RNA Nanostructures; Methods and Protocol Eckart Bindewald,Bruce A. Shapiro Book 2017 Springer Science+Business Media LLC 2017 RNA Architect

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Polycationic Probe-Guided Nanopore Single-Molecule Counter for Selective miRNA Detection,rs a potential noninvasive tool to detect miRNAs for diagnostics and prognosis applications. However, one of the challenges that limits its clinical applications is the presence of a large variety of nontarget nucleic acids in the biofluid extracts. Upon interacting with the nanopore, nontarget nucl

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Book 2017nostructures. The chapters in this book cover topics such as ion effects in RNA folding; design and crystallography of self-assembling RNA nanostructures; x-aptamer selection and validation; RNAi in HIV-infected cells; and preparation of a conditional RNA switch. Written in the highly successful .Me

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1064-3745 tion advice from the experts.Includes supplementary material.This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures. The chapters in this book cover topics such as ion effects in RNA folding; d

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A New Method to Predict Ion Effects in RNA Folding,for RNA structures. In this chapter, using homodimeric tetraloop-receptor docking as an illustrative example, we showcase the MCTBI method for the computational prediction of the ion effects in RNA folding.

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Oxime Ether Lipids as Transfection Agents: Assembly and Complexation with siRNA,and recent data from our and other groups have demonstrated that OELs are viable carriers for siRNA delivery in the cell culture systems. In this chapter, we provide the details of experimental protocols routinely used in our laboratory to examine OEL–siRNA complexes including their assembly, stability, and transfection efficiencies.