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Titlebook: RNA Nanostructures; Methods and Protocol Eckart Bindewald,Bruce A. Shapiro Book 2017 Springer Science+Business Media LLC 2017 RNA Architect

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发表于 2025-3-21 19:26:10 | 显示全部楼层 |阅读模式
书目名称RNA Nanostructures
副标题Methods and Protocol
编辑Eckart Bindewald,Bruce A. Shapiro
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts.Includes supplementary material
丛书名称Methods in Molecular Biology
图书封面Titlebook: RNA Nanostructures; Methods and Protocol Eckart Bindewald,Bruce A. Shapiro Book 2017 Springer Science+Business Media LLC 2017 RNA Architect
描述.This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures. The chapters in this book cover topics such as ion effects in RNA folding; design and crystallography of self-assembling RNA nanostructures; x-aptamer selection and validation; RNAi in HIV-infected cells; and preparation of a conditional RNA switch. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .RNA Nanostructures: Methods and Protocols. is a valuable resource for the design and production of RNA nanostructures. Researchers and scientists sharing these detailed protocols is important for sustained progress in the field..
出版日期Book 2017
关键词RNA Architectonics; 3D Structure Prediction; Exosomes; Peptides; Chrystallography
版次1
doihttps://doi.org/10.1007/978-1-4939-7138-1
isbn_softcover978-1-4939-8395-7
isbn_ebook978-1-4939-7138-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2017
The information of publication is updating

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发表于 2025-3-22 00:17:08 | 显示全部楼层
Design and Crystallography of Self-Assembling RNA Nanostructures,role in both the design and analysis of such RNA nanostructures. Here, we describe methods for the design and X-ray crystallographic structure analysis of an RNA square and two different triangles, which self-assemble from short oligonucleotides and serve as a platform for building functional nano-sized nucleic acid architectures.
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Preparation and Optimization of Lipid-Like Nanoparticles for mRNA Delivery,ate formulation optimization. This chapter focuses on the following contents relevant to lipid-like nanoparticles: formulation method, particle characterization, orthogonal array design, and in vitro assays.
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A New Method to Predict Ion Effects in RNA Folding,l ions binding to RNA can induce RNA structural changes that are important for RNA cellular functions. Therefore, quantitative modeling of the ion effects is essential for RNA structure prediction and RNA-based molecular design. Recently, inspired by the increasing experimental evidence that support
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