Titlebook: RNA Methylation; Methods and Protocol Alexandra Lusser Book 2017 Springer Science+Business Media LLC 2017 posttranscriptional base modifica

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Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencingmbination of bisulfite treatment of RNA with today’s high-throughput sequencing techniques opens the door to methylation studies at nucleotide resolution on a transcriptome-wide scale. Below we describe a protocol for the transcriptome-wide analysis of total or fractionated poly(A)RNA in cells and t

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CHOIR

High-Throughput Mapping of 2,-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMee physico-chemical methods require purification of the RNA of interest almost to homogeneity. To overcome these limitations, methods based on RT-driven primer extension have been developed and successfully used, sometimes in combination with a specific chemical treatment. Nowadays, some of these app

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Brain-Imaging

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High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-Seq)difications alter Watson-Crick base-pairing, and cause pauses or stops during reverse transcription required for most high-throughput RNA sequencing protocols, resulting in inefficient detection of methyl-modified RNAs. Here, we describe a procedure to demethylate RNAs containing m.A, m.C, or m.G us

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Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/820180.jpg

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https://doi.org/10.1007/978-1-4939-6807-7posttranscriptional base modifications; N6-methyladenosine (m6A); 5-methyl cytosine (m5C); mRNAs; long n

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Analysis of High-Throughput RNA Bisulfite Sequencing DataMethylation of the 5-cytosine (m.C) is a common but not well-understood RNA modification, which can be detected by sequencing of bisulfite-treated transcripts (RNA-BSseq). In this Chapter, we discuss computational RNA-BSseq data analysis methods for transcriptome-wide identification and quantification of m.C.

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