Titlebook: RNA Mapping; Methods and Protocol M. Lucrecia Alvarez,Mahtab Nourbakhsh Book 2014 Springer Science+Business Media New York 2014 RNA boundar

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M. Lucrecia Alvarez,Mahtab NourbakhshIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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In Situ Hybridization Detection of miRNA Using LNA™ Oligonucleotidesh combines identification of miRNAs with histochemistry (ICH). We describe in detail a protocol for ISH in formalin-fixed paraffin-embedded tissue with the help of synthetic nonradioactive LNA oligonucleotide probes.

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Mapping of Internal Ribosome Entry Sites (IRES)only be translated by a cap-independent mechanism when the intercistronic sequence is capable of ribosome binding and re-initiation of translation. Expression level of the 3′cistron is usually normalized to the expression of 5′cistron to estimate the relative IRES activity of intercistronic sequences.

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