Titlebook: RNA Interference, Editing, and Modification; Methods and Protocol Jonatha M. Gott Book 2004 Humana Press 2004

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Short Hairpin Activated Gene Silencing in Mammalian Cellsfor silencing genes. We report our results from testing a variety of shRNA design features and shRNA expression vectors. We also provide methods that use shRNAs to permit different levels of gene expression. Additionally, we discuss some aspects important for constructing an information pipeline to support development of a large shRNA library.

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Purification and Assay of Recombinant ADAR Proteins Expressed in the Yeast , or in ,DARs are described..ADARs produced in . are not enzymatically active. We describe expression of the ADAR dsRNA binding domains in . using current versions of the T7 promoter based Studier vectors as well as the purification of the domains.

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RNA InterferenceRather than ignoring such results, these researchers went on to document and further investigate the nature of such silencing, which was named “co-suppression” in plants, “quelling” in fungi, and “RNA interference” (RNAi) in nematodes. By the late 1990s, it was discovered that silencing could be ini

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Analysis of Gene Function in , Using RNA Interferencet biological phenomena. Currently, the method of choice to examine gene function in these organisms is RNA interference (RNAi). mRNA degradation is triggered by double-stranded RNA (dsRNA) produced in vivo from transgenes transcribed from opposing tetracycline (tet)-inducible T7 RNA polymerase promo

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