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Titlebook: RNA Interference and Cancer Therapy; Methods and Protocol Lekha Dinesh Kumar Book 2019 Springer Science+Business Media, LLC, part of Spring

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发表于 2025-3-21 18:34:02 | 显示全部楼层 |阅读模式
书目名称RNA Interference and Cancer Therapy
副标题Methods and Protocol
编辑Lekha Dinesh Kumar
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: RNA Interference and Cancer Therapy; Methods and Protocol Lekha Dinesh Kumar Book 2019 Springer Science+Business Media, LLC, part of Spring
描述This comprehensive volume collects a repertoire of techniques for the adoption and exploitation of RNA interference (RNAi) as a fertile strategy to develop the bio-drugs of future in the vital field of cancer research. Exploring the immense ‘bench to bedside’ potential of RNAi as a therapeutic molecule, the book focuses many of its chapters on nanoparticle-based targeted deliveries, including aptamers, as well as techniques for using RNAi technology to combat various cancers in preclinical studies. Each of the 26 chapters is authored by the pioneers in the respective areas and thus provides a practical and empirical account of the techniques that could be used as benchmark tools in the area of cancer research. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and essential, .RNA Interference and Cancer Therapy: Methods and Protocols. provides the necessary guidance for both novice and professional researchers who intend to disco
出版日期Book 2019
关键词Cancer treatment; Therapeutic tools; RNAi-based strategies; Bio-drugs; Drug development; Targeted deliver
版次1
doihttps://doi.org/10.1007/978-1-4939-9220-1
isbn_softcover978-1-4939-9222-5
isbn_ebook978-1-4939-9220-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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siRNA-Based Drug Targeting Human Bcl-xL Against Cancers, to functionally deliver tumor-specific siRNA molecules in nude mice. Here, we introduce an orthotopic tumor inoculation model in nude mice: the resulting orthotopic tumors more closely replicate the clinical conditions in humans—including the metastasis mode—than ectopic subcutaneously inoculated tumors.
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,Preparation of a Carrier to Achieve In Vivo Delivery of siRNA: The Example of Chitosan-Coated Poly(e nanoparticles with the siRNA can be achieved by performing an association yield above 90% using a mass ratio of 1 mg siRNA/50 mg of nanoparticles (20 μg siRNA/mg nanoparticles, 1 nmol siRNA (. 14 kDa)/mg nanoparticles).
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Combinatorial siRNA Polyplexes for Receptor Targeting,de a brief background on the formulation design and detailed protocols to evaluate polyplex formation, gene silencing efficiency, and receptor-directed cell killing in cancer cells using targeted combinatorial siRNA polyplexes.
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Systemic Modulation of Lymphocyte Subsets Using siRNAs Delivered via Targeted Lipid Nanoparticles,nduce therapeutic gene silencing. This approach utilizes antibodies that decorate lipid nanoparticle surfaces to home into lymphocyte subsets. This approach opens new avenues for discovery of new drug targets and potentially for therapeutics.
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