Titlebook: RNA Interference; Challenges and Thera Mouldy Sioud Book 2015 Springer Science+Business Media New York 2015 Cancer research.Extracellular m

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Magnetic Nanoparticle and Magnetic Field Assisted siRNA Delivery In Vitro,on using self-assembled complexes of small interfering RNA (siRNA) and cationic lipids or polymers that are associated with magnetic nanoparticles (MNPs). These magnetic complexes are targeted to the cell surface by the application of a gradient magnetic field. A further development of the magnetic

Culmination

Cytoplasmic Delivery of siRNAs to Monocytes and Dendritic Cells via Electroporation,lence disease genes. However, the therapeutic use of siRNA faces the in vivo delivery challenge. An alternative method that could potentially be used for siRNA delivery into primary immune cells for therapeutic purposes is an ex vivo route, whereby immune cells could be isolated from a patient, repr

Stress

Delivery of siRNAs to Cancer Cells via Bacteria,tacle for the development of RNAi-based therapeutic approaches is the delivery of the RNAi effector molecules to target cells. One promising strategy to surmount this challenge is the application of nonpathogenic bacteria as a delivery vector to target cells. In this chapter, the design of invasive

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Targeted In Vivo Delivery of siRNA and an Endosome-Releasing Agent to Hepatocytes,way: by co-opting a natural process to inhibit gene expression at the mRNA level. Given that siRNAs must interact with the cells’ natural RNAi machinery in order to exert their silencing effect, one of the most fundamental requirements for their use is efficient delivery to the desired cell type and

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Cell-Internalization SELEX: Method for Identifying Cell-Internalizing RNA Aptamers for Delivering sut RNAi-based drugs. Phase I and II studies have shown safe, strong, and durable-gene knockdown (80–90 %, lasting for a month after a single injection) and/or clinical benefit in treating several liver pathologies. Although promising, these studies have also highlighted the need for robust . techniq

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Strategies for siRNA Navigation to Desired Cells,he most serious obstacle to siRNA drug development. As the most used delivery agents can enter all cell types, specificity must be built into the delivery agents or directly attached to the siRNA molecules. The use of antibodies, peptides, Peptide-Fc fusions, aptamers, and other targeting ligands ha

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Use of Guanidinopropyl-Modified siRNAs to Silence Gene Expression, therapeutic application. To augment silencing efficacy of siRNAs, chemical modification has been employed to improve stability, target specificity, and delivery to target tissues. siRNAs incorporating guanidinopropyl (GP) moieties have demonstrated enhanced target gene silencing in cell culture and

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