Titlebook: RNA Interference; Challenges and Thera Mouldy Sioud Book 2015 Springer Science+Business Media New York 2015 Cancer research.Extracellular m

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A Facile Method for Interfering with Off-Target Silencing Mediated by the Sense Strand,ng can limit their therapeutic use. Off-targeting can be identified for both the sense and antisense siRNA strands. Some avenues of obstructing the incorporation of the sense strand into the RNA-induced silencing complex (RISC) are currently being pursued. Herein, a biotin group at the 5′-end of the

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Overcoming the Challenges of siRNA Activation of Innate Immunity: Design Better Therapeutic siRNAs, exogenously double-stranded RNA as small interfering (si) RNAs. To date, the use of siRNA (21-nt) has become a standard laboratory tool to silence gene expression in mammalian cells in-vitro and in-vivo. The methodology also holds promise for treating a diversity of human diseases. However, one of

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