Titlebook: RNA Folding; Methods and Protocol Christina Waldsich Book 2014 Springer Science+Business Media, LLC 2014 Folding pathway dissection.In vivo

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A Chemogenetic Approach to Study the Structural Basis of Protein-Facilitated RNA Folding,ve state under physiological conditions is facilitated by the protein cofactor Mss116. This chapter describes the use of Nucleotide Analog Interference Mapping (NAIM) to identify specific substructures within the intron molecule that are directly affected by the protein.

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Mapping RNA Structure In Vitro Using Nucleobase-Specific Probes,ity of all four nucleobases can be determined. With this fast and inexpensive method local changes in RNA secondary and tertiary structure, as well as the formation of contacts between RNA and its ligands can be detected independent of the RNA’s length.

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