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Titlebook: RNA Folding; Methods and Protocol Christina Waldsich Book 2014 Springer Science+Business Media, LLC 2014 Folding pathway dissection.In vivo

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书目名称RNA Folding
副标题Methods and Protocol
编辑Christina Waldsich
视频video
概述Includes cutting-edge methods and protocols for the study of RNA folding, which features in vivo techniques.Provides step-by-step detail essential for reproducible results.Contains key notes and vital
丛书名称Methods in Molecular Biology
图书封面Titlebook: RNA Folding; Methods and Protocol Christina Waldsich Book 2014 Springer Science+Business Media, LLC 2014 Folding pathway dissection.In vivo
描述.Focusing on in vitro and intracellular RNA structure formation, .RNA Folding: Methods and Protocols. provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even atomic resolution. The presented techniques include powerful tools with a long tradition in RNA research as well as more advanced, novel methods, thus the methods span multiple disciplines, including molecular biology, biochemistry, biophysics, and computational biology.  Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Practical and authoritative, .RNA Folding: Methods and Protocols. serves as a vital reference for researchers attempting to gain insights into the secrets of this astounding macromolecule.
出版日期Book 2014
关键词Folding pathway dissection; In vivo studies; Intracellular folding; RNA folding intermediates; RNA struc
版次1
doihttps://doi.org/10.1007/978-1-62703-667-2
isbn_softcover978-1-4939-6058-3
isbn_ebook978-1-62703-667-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2014
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Monitoring Global Structural Changes and Specific Metal-Ion-Binding Sites in RNA by In-line Probing structure, and function. Aside from revealing directly metal ion-binding sites these techniques also provide structural information for longer RNA sequences that are out of range to be analyzed with other techniques such as NMR. The cleavage pattern derived from in-line probing experiments reflects
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A Chemogenetic Approach to Study the Structural Basis of Protein-Facilitated RNA Folding,ne expression. In order to correctly perform its function in the cell, an RNA molecule must fold into a complex tertiary structure. Folding of many large RNAs is slow either due to formation of stable misfolded intermediates or due to high contact order or instability of obligate folding intermediat
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