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Titlebook: RNA Bioinformatics; Ernesto Picardi Book 2021Latest edition Springer Science+Business Media, LLC, part of Springer Nature 2021 Computation

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Dimensionality Reduction of Single-Cell RNA-Seq Data,cal dimensionality reduction workflow that is used for scRNA-seq datasets, specifically highlighting the roles of principal component analysis, t-distributed stochastic neighborhood embedding, and uniform manifold approximation and projection in this setting. We particularly emphasize efficient comp
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Single-Cell RNA Sequencing Analysis: A Step-by-Step Overview, a single experiment. Since its introduction, single-cell RNA sequencing (scRNA-seq) approaches have revolutionized the genomics field as they created unprecedented opportunities for resolving cell heterogeneity by exploring gene expression profiles at a single-cell resolution. However, the rapidly
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Advanced Design of Structural RNAs Using RNARedPrint,ructures. Depending on the application, these structures could represent alternate configurations or kinetic pathways. The software makes such design convenient and sufficiently fast for practical routine, where it even overcomes notorious problems in the application of RNA design, e.g., it maintains realistic . content.
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Statistical Modeling of High Dimensional Counts,orming data input, scaling/normalization, visualization, and statistical testing to determine sets of features where the counts reflect differences in gene expression across samples. Finally, I discuss limitations and possible extensions to the models presented here.
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Normalization of Single-Cell RNA-Seq Data,mers. Here, we show how to use R/Bioconductor to calculate normalization factors, apply them to compute normalized data, and compare several normalization approaches. Finally, we briefly show how to perform downstream analysis steps on the normalized data.
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