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Titlebook: RNA Activation; Long-Cheng Li Book 2017 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singap

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miRNA-Mediated RNAa by Targeting Enhancers miRNAs are also distributed in the nucleus, with its function largely undetermined. At the same time, while miRNAs and enhancers show obvious tissue specificity, the interaction between miRNAs and enhancers in gene regulation remains unknown. By screening miRNA databases, we have identified a subse
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Specific Increase of Protein Levels by Enhancing Translation Using Antisense Oligonucleotides Target interest. Protein expression is downregulated by some structural or sequence elements present in the 5′ UTR of mRNAs, such as upstream open reading frames (uORF). Translation initiation from uORF(s) reduces translation from the downstream primary ORF encoding the main protein product in the same mR
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Repurposing CRISPR System for Transcriptional Activationlar one for genome editing. When the nuclease domains of Cas9 protein are mutated into deactivated form (dCas9), CRISPR/dCas9 still retains the ability to bind the targeted DNA sequence, but loses the endonuclease cleavage activity. Taking advantage of the characteristics of this engineered nuclease
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RNA-Mediated Gene Activation: Identifying a Candidate RNA for Preclinical Developmenton of a therapeutic protein. Duplex RNAs that target gene promoters are one strategy for enhancing the expression of target genes. This chapter summarizes our understanding of mechanism behind gene activation by small RNAs that bind noncoding transcripts at gene promoters. We describe a path for cho
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Enhancing Angiogenesis in Mice by VEGF-Targeting Small Activating RNAsene therapy are needed. During the last decade, the role of small noncoding RNAs (ncRNAs) in the regulation of gene expression at the transcriptional level has been shown. Promoter-targeted small RNAs recruit histone-modifying enzymes and can either repress or induce target gene expression. As an ex
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