Titlebook: RNA; Methods and Protocol Henrik Nielsen Book 2011 Springer Science+Business Media, LLC 2011 Bioinformatics.Mammalian genome.Messenger RNAs

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Inhibition of Gene Function in Mammalian Cells Using Short-Hairpin RNA (shRNA)RNAi is now the preferred method for silencing gene expression in a variety of systems. In this chapter we describe the procedure for applying short-hairpin RNA (shRNA) to study gene function. Detailed descriptions of target site selection, shRNA construction, shRNA transfection and target knockdown validation are included.

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Knud Josefsen,Henrik Nielseny different contributors. The author,together with colleague authors who provide valuable additionalexperience, presents examples of actual industrial world applications.Furthermore the academic concepts presented in this book provideexcellent theories to student readers and the concepts described a

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