Titlebook: Quantum Dots; Applications in Biol Adriana Fontes,Beate S. Santos Book 2020Latest edition Springer Science+Business Media, LLC, part of Spr

经典

Measuring the Hydrodynamic Radius of Colloidal Quantum Dots by Fluorescence Correlation Spectroscopye-based techniques, for over two decades. A great variety of QD syntheses and conjugations are available, and tailoring these for the desired application requires a refined structural characterization. Life science applications rely on the interaction of QDs with biostructures; hence, the knowledge

Chandelier

Encapsulating Quantum Dots in Lipid–PEG Micelles and Subsequent Copper-Free Click Chemistry Bioconjuapolar organic solvents to water, the optical properties of the fluorescent nanoparticles must be maintained; additionally, the resulting colloid should be monodisperse and stable against aggregation. Furthermore, the hydrophilic coating should confer functional groups or conjugation handles to the

Facet-Joints

散布

Phase Transfer and DNA Functionalization of Quantum Dots Using an Easy-to-Prepare, Low-Cost Zwitteriaining these characteristics can be highly specialized or expensive. We describe a straightforward, low-cost protocol for functionalizing poly(isobutylene-.-maleic anhydride) (PIMA) with moieties that anchor to the QD surface (histamine), impart hydrophilicity [(2-aminoethyl)trimethylammonium chlori

一美元

Dextran-Functionalized Quantum Dot Immunoconjugates for Cellular Imagingerties and surface area that supports multivalent conjugation of biomolecules. An important design consideration for effective use of these materials is a hydrophilic, biocompatible surface chemistry that provides colloidal stability and minimizes nonspecific interactions with biological molecules a

osteopath

Labeling Neuronal Proteins with Quantum Dots for Single-Molecule Imaginges in the brain. Here, we describe how nanometer-sized fluorescent semiconductors called quantum dots (QD) can be used to label neuronal proteins in a single QD imaging format. We detail two generalizable protocols accompanied by experimental considerations giving the user options in approach tailor

叫喊

Quantitative Chemical Delivery of Quantum Dots into the Cytosol of Cellsn attractive solution to this problem due to their exceptional photostability and brightness. Here, we describe in detail a protocol to chemically deliver functionalized QDs into the cytosol of living cells, based on cell-penetrating poly(disulfide)s (CPDs). This protocol is highly efficient and del

挣扎

Conscientious

Resazurin-Based Assay to Evaluate Cell Viability After Quantum Dot Interactiones in cell viability using a variety of methods, especially colorimetric ones. One of the most applied tests is the MTT assay. In comparison to MTT, for example, the resazurin-based method has the main advantage of not evaluating the cells directly, thus eliminating false-positive results that may a

小步走路