Titlebook: Quantitative Methods in Proteomics; Katrin Marcus Book 2012 Springer Science+Business Media, LLC 2012 MS-based methods.enzymatic labeling.

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Fermentation

Relative Protein Quantification by MS/MS Using the Tandem Mass Tag Technologyand ExacTag are examples of such technology. Experimental design, sample preparation and separation, MS acquisition parameters, and data analysis are the key steps to achieve accurate and precise quantitative measurements. We describe herein an isoelectric focusing shotgun proteomics workflow for th

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CHYME

Isotope-Coded Protein Label in the different proteomic states are further analyzed by tandem-mass spectrometry to identify respective proteins. For quantification of proteins from multiplexed ICPL experiments, . was developed, a software package especially designed to cover the whole ICPL workflow. The ICPL method results in

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Hydroponic Isotope Labeling of Entire Plants and High-Performance Mass Spectrometry for Quantitativetrometer, combining high mass accuracy and sensitivity for the MS data acquisition with speed and high-throughput MS/MS data acquisition, increasing the number of proteins identified and quantified and improving protein quantitation. Peak processing and picking from raw MS data files, protein identi

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Post-digestion 18O Exchange/Labeling for Quantitative Shotgun Proteomics of Membrane Proteins for quantitative profiling of the detergent-insoluble membrane proteins isolated from HeLa cells, differentially transfected with plasmids expressing HIV Gag protein and its myristylation-defective N-terminal mutant. Whilst this protocol depicts solubilization of detergent-insoluble membrane protei

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Robust Workflow for iTRAQ-Based Peptide and Protein Quantificationic digestion protocol, (c) SPE desalting and subsequent peptide labeling using a 4-plex iTRAQ labeling kit, and (d) fractionation of the obtained peptide mixture by strong cation exchange chromatography.