Titlebook: Quantitative Methods in Proteomics; Katrin Marcus Book 2012 Springer Science+Business Media, LLC 2012 MS-based methods.enzymatic labeling.

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Important Issues in Planning a Proteomics Experiment: Statistical Considerations of Quantitative Proimes neglected part in detecting differential proteins is the statistical analysis. Data from proteomics experiments are usually high-dimensional and hence require profound statistical methods. It is especially important to already correctly design a proteomic experiment before it is conducted in th

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Proteome Analysis with Classical 2D-PAGEon, proteins are separated by their isoelectric point, a technique known as isoelectric focusing (IEF). There are two important variants of IEF, which are carrier-ampholine (CA)-based IEF and immobilized pH gradient (IPG)-based IEF. In the second dimension, proteins are further separated by their el

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Fast and Sensitive Coomassie Staining in Quantitative Proteomicsould bind non-covalently to the protein following a linear response curve. Since protein concentrations in biological systems may vary by six or more orders of magnitude (Corthals GL et al., Electrophoresis 21(6):1104–1115, 2000), the staining should allow for a detection of very low protein amounts

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