Titlebook: Quantitation of mRNA by Polymerase Chain Reaction; Nonradioactive PCR M Thomas Köhler,Dirk Laßner,Barbara Pustowoit Book 1995 Springer-Verl

BARB

cardiovascular

Synthesis of cDNAFor detection or quantitation of mRNA by polymerase chain reaction, it is necessary to transcribe mRNA into copy-DNA (cDNA) by a reverse transcriptase (RT) reaction. The quality of a cDNA depends on the integrity of the messenger RNA and the fidelity of the transcription.

不可比拟

clarify

AGGER

博识

Quantitation of Absolute Numbers of mRNA Copies in a cDNA Sample by Competitive PCRIn this chapter a competitive PCR assay using an internal DNA standard is described. Competitive PCR is a quantitative adaptation of the PCR method in which a known number of copies of an exogenous synthesized and added DNA (or RNA) is amplified together with the sample in the same PCR tube (see chapter 1.1).

旧式步枪

Muffle

合唱队

archetype