Titlebook: Quantification of Tannins in Tree and Shrub Foliage; A Laboratory Manual Harinder P. S. Makkar Book 2003 Springer Science+Business Media Do

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Book 2003nderstand manner. This unique guide covers chemical, biological and radio isotopic tannin assays. Comprehensive step-by-step protocols are presented for each method. The protocols enable non-specialists and specialists alike to implement the methods easily in the laboratory. It is an ideal laborator

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Treatment of Plant Material, Extraction of Tannins, and an Overview of Tannin Assays Presented in tins), and are considered to have both adverse and beneficial effects depending on their concentration and nature besides other factors such as animal species, physiological state of the animal and composition of the diet.

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Summary of Results from the First Phase of the Co-Ordinated Research Project,alidation of tannin assays to seek correlation with animal performance indicators; and b) plan future studies. It was attended by 10 Research Contract and Agreement holders, the Scientific Secretary of the Project, and 10 staff members and students of the host institution.

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Book 2003or each method. The protocols enable non-specialists and specialists alike to implement the methods easily in the laboratory. It is an ideal laboratory manual for research scientists, graduate students, and laboratory personnel working in the fields of animal nutrition, soil nutrient management, wild life-plant interactions, and plant breeding.

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Radial Diffusion Assay,Type I, gel point 36°C (Sigma A-6013), bovine serum albumin (fraction V) (Sigma A-3350), glacial acetic acid, ascorbic acid and sodium hydroxide.

观察

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Radiolabelled BSA Precipitation Method,This is the most sensitive method for determining amounts of protein precipitated by a tannin. It is also useful for running competitive binding assay to characterize tannin-protein interactions. The method described here [26] has been scaled down from the original method [27].

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