Titlebook: piRNA; Methods and Protocol Nicholas F. Parrish,Yuka W. Iwasaki Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive

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CRISPR-Mediated Genome Engineering in r mosquito, ., is one such organism. It is also responsible for transmitting a wide variety of deadly viruses including Dengue, Zika, Yellow fever, and Chikungunya. The key to successful CRISPR-mediated gene editing applications is the delivery of both Cas9 ribonuclease and single-guide RNA (sgRNA)

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PIWI-Directed DNA Elimination for Tetrahymena Geneticsgrammed to induce ectopic DNA elimination at basically any given genomic location. Here, we describe the usage of codeletion for genetic studies in . and for investigations of the molecular mechanism of Piwi-directed programmed DNA elimination.

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Isolation and Processing of Bovine Oocytes for Small RNA Sequencingications. Over the last years, it has become clear that female mice employ an exceptional piRNA pathway-independent mechanism to neutralize transposon activity in the ovary. In other model organisms studied to date, the piRNA pathway is indispensable for efficient targeting of transposable elements

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3D Imaging and In Situ Hybridization for Uncovering the Functions of MicroRNA in Rice Anther is enriched during reproduction in grasses, is a trigger to produce phased small interfering RNAs (phasiRNAs). These phasiRNAs demonstrate the temporal regulation with premeiotic phasiRNAs and meiotic phasiRNAs in anther development. Furthermore, the site-specific regulation via miR2118 and phasiRN

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Drosophila Genetic Resources for Elucidating piRNA Pathwayretrotransposon silencing in metazoans. Numerous studies have characterized the mechanism of retrotransposon silencing and identified dozens of factors involved in the piRNA pathways. . is an attractive model organism for piRNA studies due to its great availability of genetic tools and the low cost

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