Titlebook: piRNA; Methods and Protocol Nicholas F. Parrish,Yuka W. Iwasaki Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive

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Whole-Mount RNA FISH Combined with Immunofluorescence for the Analysis of the Telomeric Ribonucleoproteins in the , Germline protein of interest simultaneously. Although whole-mount RNA FISH is routinely used to determine RNA intracellular localization, a detailed picture of RNA distribution in complex tissues remains a challenge. The main problem is the various permeability of morphologically different cells within a ti

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Solid-Support Directional (SSD) RNA-Seq as a Companion Method to CLIP-Seqeins (RBPs), as it can scrutinize the RNAs bound by an RBP in vivo, with minimum manipulation of biological samples. CLIP-Seq is best used to reveal changes of the RNA cargo of an RBP and differences on binding patterns of the bound RNAs in living cells in different genetic backgrounds or after expe