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Titlebook: p53 Protocols; Sumitra Deb,Swati Palit Deb Book 2003 Humana Press 2003

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Interaction of p53 with the Adenovirus E1B-55 kDa Protein,raction of E1B-55 kDa with p53; (.) inhibition of p53-induced transcription; (.) relocalization of p53 from the nucleus to the cytoplasm; and (.) in the simultaneous presence of E1B-55 kDa and the adenovirus E4-34 kDa (E4orf6) protein, extensive destabilization of p53. These activities can be observ
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Analysis of Nuclear and Cytoplasmic Degradation of p53 in Cells after Stress, nuclear export of p53 via the CRM1 pathway. However, proteasomes are abundant in both cytosol and nucleus. We recently analyzed HDM2-mediated degradation of endogenous p53 in the presence of various CRM1 blockers. We found that significant HDM2-mediated degradation takes place despite nuclear expor
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Mutational Analysis of p53 in Human Tumors,d from the tumor samples and then used as a template to amplify the p53 coding sequences by polymerase chain reaction (PCR). The PCR products are characterized by agarose gel electrophoresis. Subsequently, mutations can be detected by direct DNA sequencing of the PCR products, followed by acrylamide
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