书目名称 | Protocols for Neural Cell Culture |
编辑 | Sergey Fedoroff,Arleen Richardson |
视频video | http://file.papertrans.cn/762/761965/761965.mp4 |
图书封面 |  |
描述 | This volume is designed to be kept close at hand as a ready reference and a guide to laboratory procedures. It is based on tissue culture manuals used for a number of years at international courses on tissue culture at the University of Saskatchewan, made possible by the generous support of the Canadian Council of Animal Care and the Medical Research Council of Canada. Sergey Fedoroff Arleen Richardson The second edition of Protocols for Neural Cell Culture adheres to the prin ciples enunciated in the first edition, but the content has been extensively revised and expanded. Two new chapters have been added to reflect the increased interest in the development and differentiation of the nervous system and in the reconstruction of its circuitry in tissue culture. One chapter deals with slice cul tures in which the organization of the nervous system is preserved. When slice cultures are combined with explant cultures, afferent and efferent projections can be reconstructed. The other chapter deals with aggregating neural cell cul tures, in which "minibrains" can form. Theses are small, uniformly sized spheres of nervous tissue, usually having nerve cells in the center and astrocytes, |
出版日期 | Book 19972nd edition |
关键词 | Nervous System; brain; cell; cell culture; development; gene; gene therapy; genes; glia; neurons; tissue |
版次 | 2 |
doi | https://doi.org/10.1007/978-1-4757-2586-5 |
isbn_ebook | 978-1-4757-2586-5 |
copyright | Springer Science+Business Media New York 1997 |