书目名称 | Protein Gel Detection and Imaging |
副标题 | Methods and Protocol |
编辑 | Biji T. Kurien,R. Hal Scofield |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | This volume expands upon the collection of techniques published in .Protein Electrophoresis: Methods and Protocols .(2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Comprehensive and practical, .Protein Gel Detection and Imaging: Methods and Protocols. is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field.. |
出版日期 | Book 2018 |
关键词 | Polyacrylamide Gels; Coomassie Blue; imidazole-zinc; PAGE; Metalloproteins |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-8745-0 |
isbn_softcover | 978-1-4939-9381-9 |
isbn_ebook | 978-1-4939-8745-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media, LLC, part of Springer Nature 2018 |