| 书目名称 | Pollen and Pollen Tube Biology |
| 副标题 | Methods and Protocol |
| 编辑 | Anja Geitmann |
| 视频video | |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | .This volume explores a collection of experimental techniques used to investigate different aspects of pollen development and function, including its role in reaching the ovule and delivering the two sperm cells. The techniques discussed range from basic methodology to cultivate pollen in vitro to the sophisticated experiments involving micromanipulation, Lab-on-Chip technology, or high-end imaging. The chapters in this book cover topics such as pollen grain counting using a cell counter; restricted pollination for tracing individual pollen tubes in a pistil; obtaining mutant pollen for phenotypic analysis and pollen tube dual-staining; analyzing intracellular gradients in pollen tubes; and measuring exocytosis rate in Arabidopsis pollen tube using corrected fluorescence recovery after photoconversion (cFRAPc) technique. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Pollen and Pollen Tube Biology: Methods a |
| 出版日期 | Book 2020 |
| 关键词 | gametophyte; Bimolecular fluorescence; Genome-wide association study (GWAS); RStudio; RNA-Seq expression |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-0716-0672-8 |
| isbn_softcover | 978-1-0716-0674-2 |
| isbn_ebook | 978-1-0716-0672-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media, LLC, part of Springer Nature 2020 |
发表于 2025-3-21 17:41:59
