| 书目名称 | Plant Proteomics |
| 副标题 | Methods and Protocol |
| 编辑 | Hervé Thiellement,Michel Zivy,Valérie Méchin |
| 视频video | http://file.papertrans.cn/749/748789/748789.mp4 |
| 概述 | Includes supplementary material: |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapt |
| 出版日期 | Book 2007 |
| 关键词 | Microarray; Polyacrylamid-Gelelektrophorese; Proteomics; Seed; Translation; arabidopsis thaliana; membrane |
| 版次 | 1 |
| doi | https://doi.org/10.1385/1597452270 |
| isbn_softcover | 978-1-61737-686-3 |
| isbn_ebook | 978-1-59745-227-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Humana Press 2007 |
|Archiver|手机版|小黑屋|
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