书目名称 | Plant Phosphoproteomics | 副标题 | Methods and Protocol | 编辑 | Waltraud X. Schulze | 视频video | | 概述 | Includes cutting-edge methods and protocols in plant phosphoproteomics.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This detailed volume addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system. Over the recent decades, proteomic methods were refined to study the significance and dynamics of protein phosphorylation in various biological contexts. However, working with plant tissue imposes particular challenges to the biologist which are attributed to the rigid cell wall making protein extraction more difficult, the skewed protein abundance with Rubisco as a highly abundant protein and a large central vacuole leading to low protein yield and increased degradative enzyme activity. The methodologies in this book seek to move beyond these issues. Written for the .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Practical and authoritative, .Plant Phosphoproteomics: Methods and Protocols. serves as an ideal reference for researchers investigating this vital area of plant science.. | 出版日期 | Book 2015 | 关键词 | Kinases; Phosphoproteomic techniques; Phosphorylation; Plant system; Plant tissue; Posttranslational modi | 版次 | 1 | doi | https://doi.org/10.1007/978-1-4939-2648-0 | isbn_softcover | 978-1-4939-4937-3 | isbn_ebook | 978-1-4939-2648-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media New York 2015 |
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