书目名称 | Plant MicroRNAs | 副标题 | Methods and Protocol | 编辑 | Blake C. Meyers,Pamela J. Green | 视频video | | 概述 | Presents fundamentals of plant miRNA biology, including pathways and approaches used for their discovery and investigation, as written by leading researchers in the field.Covers a broad range of molec | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In .Plant MicroRNAs: Methods and Protocols., experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful .Methods in Molecular Biology™. series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls...Authoritative and easy to use, .Plant MicroRNAs: Methods and Protocols. provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typi | 出版日期 | Book 2010 | 关键词 | Arabidopsis; Evolution; Heterochromatic siRNAs; Post-transcriptional regulatory effects; Promoter; RNA pu | 版次 | 1 | doi | https://doi.org/10.1007/978-1-60327-005-2 | isbn_softcover | 978-1-61779-653-1 | isbn_ebook | 978-1-60327-005-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2010 |
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