书目名称 | Plant MAP Kinases |
副标题 | Methods and Protocol |
编辑 | George Komis,Jozef Šamaj |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of .Plant MAP Kinases: Methods and Protocols. is to provide established and new MAPK protocols adapted to the challenges posed by working with plants. The book contains 19 chapters which encompass a wide array of methods progressively scaling from the single gene, protein or cell level to large-scale arrays of proteomic, phosphoproteomic and interactomic data in order to uncover previously unidentified plant MAPK signaling pathways and to tackle with the challenging task of substrate identification. Techniques for MAPK sequence analysis and subcellular localization helping to identify their substrates and subcellular compartmentalization are also provided. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding know |
出版日期 | Book 2014 |
关键词 | Arabidopsis mesophyll protoplasts; MAPK phosphorylation/activation; MAPK transcriptional regulation; fu |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-0922-3 |
isbn_softcover | 978-1-4939-4589-4 |
isbn_ebook | 978-1-4939-0922-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2014 |