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Titlebook: Parasite Genomics Protocols; Sara E. Melville Book 2004 Humana Press 2004

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发表于 2025-3-21 18:55:47 | 显示全部楼层 |阅读模式
书目名称Parasite Genomics Protocols
编辑Sara E. Melville
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Parasite Genomics Protocols;  Sara E. Melville Book 2004 Humana Press 2004
描述Parasitic diseases remain a major health problem throughout the world, for both humans and animals. For many of us, our technologically advanced lifestyle has decreased the prevalence and transmission of parasitic diseases, but for the majority of the world’s population, they are ever present in homes, domestic animals, food, or the environment. The study of parasites and parasitic disease has a long and distinguished history. In some cases, it has been driven by the great importance of the presence of the parasite to the community, for example, those that affect our livestock. In other cases, it is clear that applied research has suffered for lack of funding because the parasite affects people with few resources, such as the rural poor in resource-poor countries. These instances include the so-called “neglected diseases,” as defined by the World Health Organization (WHO). Parasites have complicated life cycles, and a thorough understanding of the unique characteristics of a particular parasite species is vital in attempts to avoid, prevent, or cure infection or to alleviate symptoms. Of course, the biological characteristics that each parasite has developed to aid survival and tra
出版日期Book 2004
版次1
doihttps://doi.org/10.1385/1592597939
isbn_softcover978-1-61737-331-2
isbn_ebook978-1-59259-793-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2004
The information of publication is updating

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发表于 2025-3-21 23:53:19 | 显示全部楼层
Analysis of Differentially Expressed Parasite Genes and Proteins Using Transcriptomics and Proteomics,acrylamide gel electrophoresis. These two methods are then integrated to allow the global changes in RNA and protein expression to be examined. The protocols have been adapted for working on parasites and contain extensive notes.
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1064-3745 te species is vital in attempts to avoid, prevent, or cure infection or to alleviate symptoms. Of course, the biological characteristics that each parasite has developed to aid survival and tra978-1-61737-331-2978-1-59259-793-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
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RACE and RAGE Cloning in Parasitic Microbial Eukaryotes, full-length mitochondrial Hsp70 could be cloned and sequenced and specific antibody raised against a fusion protein. The highly specific antibody allowed us to demonstrate for the first time the presence of mitochondrial-like organelles in microsporidia.
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Random Mutagenesis Strategies for Construction of Large and Diverse Clone Libraries of Mutated DNA Fragments,from the reaction by . digestion, the pool of mutagenized plasmids can then be used directly in screening procedures..The different random mutagenesis strategies we describe should have wide applications in the production of libraries of large and diverse DNA libraries and in the generation of mutant proteins for structural and functional studies.
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