Titlebook: Oxidative Stress Biomarkers and Antioxidant Protocols; Donald Armstrong Book 2002 Humana Press 2002

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Oxidative Stress Biomarkers and Antioxidant Protocols978-1-59259-173-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Donald ArmstrongIncludes supplementary material:

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Human Xanthine Oxidoreductase Determination by a Competitive ELISAXanthine oxidoreductase (XO) catalyses the oxidation of hypoxanthine to xanthine and of the latter to uric acid. The enzyme is present in traces in most of human tissues, including plasma, being more abundant in milk, liver, and intestine (.).

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Mass Spectrometric Quantification of F2-Isoprostanes as Indicators of Oxidant Stressis, cancer, and neurodegenerative disorders (.). Much of the evidence for this association, however, is indirect largely because of limitations in techniques currently available to quantify free radicals or their products in biological systems (.,.). This is a particular problem when assessing oxidant injury in vivo in humans.

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Detection of Certain Peroxynitrite-Induced DNA Modificationste is mediated by an intermediate with the biological activity of the hydroxyl radical. However, this product does not appear to be the hydroxyl radical ., but peroxynitrous acid (ONOOH) or its activated isomer (ONOOH*) (.). Peroxynitrite readily reacts with proteins, lipids, and DNA under conditions of inflammation.

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Methods in Molecular Biologyhttp://image.papertrans.cn/o/image/705305.jpg

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Simultaneous Determination of Polyunsaturated Fatty Acids and Corresponding Monohydroperoxy and Monos the 1,4-pentadiene structure of a polyunsaturated fatty acid (PUFA), which are either free or esterified to cholesterol or glycerol. Initiation occurs when a FR abstracts a methylene hydrogen from PUFA. In this reaction the FR is quenched and a PUFA centered alkoxyl radical (L•) is formed. L• then

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Determination of Products of Lipid Oxidation by Infrared Spectroscopytype of oxidant, and severity of the oxidation, a variety of lipid-peroxidation products are formed (.). The major proucts of lipid peroxidation are moieties containing hydroxyls, hydroperoxyls, aldehydes, ketones, caroxylic acids, and . double bonds. Infrared spectroscopy is a sensitive technique t

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Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodiesducts such as aldehydes can easily react with biomolecules such as proteins (.). The aldehyde-derived adduct formation has been examined by many researchers using chemical and immunochemical methods (.-.). However, on the other hand, the lipid hydroperoxide itself might react with protein as already