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Titlebook: Ovarian Cancer; Methods and Protocol Pamela K. Kreeger Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t

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发表于 2025-3-21 19:22:27 | 显示全部楼层 |阅读模式
书目名称Ovarian Cancer
副标题Methods and Protocol
编辑Pamela K. Kreeger
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Ovarian Cancer; Methods and Protocol Pamela K. Kreeger Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t
描述This detailed volume provides a robust set of methods to understand variation between patients with ovarian cancers, in vitro models to better study different stages of the disease, and in vivo models to test therapies. Beginning with clinical perspectives to orient basic scientists, the book continues with sections exploring methods to characterize features of the tumor microenvironment (TME), techniques to isolate cells for in vitro study, and in vitro and in vivo models to study the disease. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Ovarian Cancer: Methods and Protocols. serves as an ideal guide for researchers seeking to improve their study of ovarian cancer and find newtherapeutic approaches..
出版日期Book 2022
关键词Tumor microenvironment; Cell isolation; Ovary; Fallopian tube; Cancer cell lines; Tumor development; Thera
版次1
doihttps://doi.org/10.1007/978-1-0716-1956-8
isbn_softcover978-1-0716-1958-2
isbn_ebook978-1-0716-1956-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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978-1-0716-1958-2The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Ovarian Cancer978-1-0716-1956-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
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https://doi.org/10.1007/978-1-0716-1956-8Tumor microenvironment; Cell isolation; Ovary; Fallopian tube; Cancer cell lines; Tumor development; Thera
发表于 2025-3-22 16:09:15 | 显示全部楼层
Quantitative Analysis of the Extracellular Matrix by Immunoblot healthy and diseased tissue. Here we describe a method of integrating quantitative dot blot along with immunohistochemistry on the same patient sample in order to calculate the overall protein concentration as well as the concentration in a region of interest.
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Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumorsid tissues, specifically freshly resected tubo-ovarian high-grade serous tumors. Here we detail our protocols designed to ensure the production of high-quality single-cell datasets. The methodology can be modified to accommodate the study of other solid tissues.
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