Titlebook: Organoids; Stem Cells, Structur Kursad Turksen Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Regenerative me

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1064-3745 ily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Comprehensive and cutting-edge, .Organoids: Stem Cells, Structure, and Function. serves as an aid to researchers working in this vital area of research..978-1-4939-7617-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

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A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment,r-free method of generating cerebral organoids from feeder-dependent or feeder-free human pluripotent stem cells using standard laboratory equipment. The protocol outlined below allows generation of 3D tissues, which replicate human early in vivo brain development up to the end of the first trimeste

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Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres,y minimal cell growth and differentiation. Here, we report a protocol that dramatically increases the long-term expansion of primary human airway basal cells while maintaining their genomic stability using 3T3-J2 fibroblast coculture and ROCK inhibition. We also describe techniques for the different

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Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix,ure system, organoids with multilayered squamous keratinized epithelium and typical morphological features of filiform papillae are generated from single lingual epithelial cells at a high efficiency. The culture system is created in order to observe the differentiation and maturation process of eac

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Generation of Functional Kidney Organoids In Vivo ,tarting from a Single-Cell Suspension,gans and are termed organoids. In this chapter we describe a technique using suspensions of fully dissociated mouse kidney cells to yield organoids that can become vascularized in vivo and mature and display physiological functions. This system can be used to produce fine-grained human–mouse chimeri

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