Titlebook: Organoids; Stem Cells, Structur Kursad Turksen Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Regenerative me

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The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine, When fragments of epithelial tissue released by incubation with EGTA (2.5 mM, 2 h) are embedded in Matrigel matrix on cell culture inserts the formation of empty spheres covered by epithelial cells is observed in first 24 h of culture. The growth and survival of organoids are supported by the addit

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,Intestinal Organoids as a Novel Tool to Study Microbes–Epithelium Interactions,icrobiota and the host is symbiotic and mutualistic, influencing many aspects of the biology of the host. This homeostatic balance can be disrupted by enteric pathogens, such as . or ., which are able to invade the epithelial layer and consequently subvert physiological functions. To study the host–

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Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observ The growth of ISCs into crypt organoids provides an in vitro model for studying the mucosal physiology, intestinal cancer tumorigenesis, and intestinal regeneration. Here, we describe two different isolation protocols and demonstrate a fixation method that aids in the confocal observation of the or

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