Titlebook: Optical Imaging of Neocortical Dynamics; Bruno Weber,Fritjof Helmchen Book 2014 Science+Business Media New York 2014 Cortical processing.F

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In Vivo Population Imaging of Dendritic Integration in Neocortexe, thus allowing the fluorescence in these dendrites to be isolated from all other filled structures. Here we describe the details of this approach (also called the “periscope” method) and give examples of the kinds of recordings that can be made with this approach.

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Laser Speckle Imaging of Cerebral Blood Flowo-)physiological aspects of neurovascular coupling. LSI is a rather simple and cost-effective method to acquire two-dimensional maps of cerebral blood flow from the cortex of experimental animals. In this chapter, we introduce the physical underpinnings as well as the most important technical and practical aspects of its implementation.

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Voltage-Sensitive Dye Imaging of Cortical Dynamics behaving mice reveals interactions between somatosensory and motor cortex that depend strongly on behavioral state. The recent development of genetically encoded voltage-sensitive fluorescent proteins will likely help towards long-term measurements of the electrical activity of specific cell types.

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Imaging of Astrocytic Activity in Living Rodentsto monitor Ca. concentration fluctuations in a population of astrocytes or in astrocytic processes using in vivo two-photon microscopy. Simultaneous recording of neuronal activity by electrodes and astrocytic activity by imaging is a promising way to reveal the nature of neuron–glia interactions in the brain of living rodents.

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Two-Photon Microscopy to Measure Blood Flow and Concurrent Brain Cell Activityts and mice, determine the receptive fields of the exposed cortical areas, and use two-photon microscopy to accurately measure blood flow in individual cortical vessels concurrent with local cellular activity. We illustrate the techniques with acute recordings from rats and chronic recordings from mice.

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0893-2336 states.Features detailed techniques from the labs of expert.The application of optical methods for investigating neocortical circuit dynamics has greatly expanded in recent years, providing novel insights into the fascinating world of brain function. .Optical Imaging of Neocortical Dynamics. presen

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Neocortex in the Spotlight: Concepts, Questions, and Methods information and memorized experiences and they constantly adapt to outside challenges through learning. These cognitive capabilities largely arise from neural processing in the outermost thin sheet of the forebrain called the neocortex. Although the mammalian neocortex has been studied extensively,