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Titlebook: Opioid Research; Methods and Protocol Zhizhong Z. Pan Book 2003 Humana Press 2003

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发表于 2025-3-21 16:56:26 | 显示全部楼层 |阅读模式
书目名称Opioid Research
副标题Methods and Protocol
编辑Zhizhong Z. Pan
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Medicine
图书封面Titlebook: Opioid Research; Methods and Protocol Zhizhong Z. Pan Book 2003 Humana Press 2003
描述Opioid research is one of the multidisciplinary research areas that involve advanced techniques ranging from molecular genetics to neuropharmacology, and from behavioral neuroscience to clinical medicine. In current opioid research, it has become increasingly important to use multiple approaches at molecular, cellular, and system levels for investigations on a specific opio- related target system. That often requires understanding and applying cro- field techniques and methods for the success of one’s research projects. Through its broad spectrum of coverage, Opioid Research: Methods and Protocols provides a comprehensive collection of major laboratory methods and protocols in current opioid research, covering topics from molecular and genetic techniques to behavioral analyses of animal models, and then to clinical practice. It will serve as a convenient reference book from which those involved in opioid research will learn or perfect the necessary cross-field techniques. The detailed methods and protocols described in Opioid Research: Methods and Protocols have each been successfully applied in current opioid research. Part I provides molecular techniques for the cloning and expre
出版日期Book 2003
版次1
doihttps://doi.org/10.1385/1592593798
isbn_softcover978-1-61737-329-9
isbn_ebook978-1-59259-379-8Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightHumana Press 2003
The information of publication is updating

书目名称Opioid Research影响因子(影响力)




书目名称Opioid Research影响因子(影响力)学科排名




书目名称Opioid Research网络公开度




书目名称Opioid Research网络公开度学科排名




书目名称Opioid Research被引频次




书目名称Opioid Research被引频次学科排名




书目名称Opioid Research年度引用




书目名称Opioid Research年度引用学科排名




书目名称Opioid Research读者反馈




书目名称Opioid Research读者反馈学科排名




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Assessing Opioid Regulation of Adenylyl Cyclase Activity in Intact Cellsse is commonly exploited to study the responsiveness to opioid ligands at the cellular level. In most of the cell systems studied, acute activation of opioid receptors leads to inhibition of adenylyl cyclase activity and a decrease in intracellular cAMP levels (.).
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Immunohistochemical Localization of μ-, δ- and K-Opioid Receptors Within the Antinociceptive Brainsttinociception. It has been hypothesized that the analgesic effects of opiates are mediated indirectly through inhibition of inhibitory GABAergic neurons within the PAG-RVM-spinal cord circuit (.,.–.).
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Study of Opioid Receptor Phosphorylation Using Cell-Labeling Method with 32P-Orthrophosphate charged phosphate group alters the structure of the protein and regulates its functional properties. Protein phosphorylation is a highly regulated reversible process and thus the level of receptor phosphorylation in neurons is constantly modified by neurotransmitters and other cellular signals released by surrounding neurons.
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Hybridization in Neural Tissuese or nonradioactive techniques and quantitated using specialized image analysis programs. This chapter will describe a sensitive radioactive method of . hybridization, with references given to other variants in technique.
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Opioid Receptor Oligomerizationby the activation of multiple signal transduction pathways including the inhibition of adenylyl cyclase, modulation of inwardly rectifying K. channels or voltage-dependent calcium channels and regulation of mitogen-activated protein (MAP) kinase activity (.–.).
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