Titlebook: Opioid Research; Methods and Protocol Zhizhong Z. Pan Book 2003 Humana Press 2003

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reiheit ‘84 — Macht oder Ohnmacht der Medien?” Der Saal war überfüllt. Auf dem Podium saßen Politiker, Journalisten, Wissenschaftler. Rechts neben mir der damalige Korrespondent des ZDF in Paris, Horst Schättle. Wir langweilten uns und begannen ein Gespräch über mein Lieblingsthema zur damaligen Zei

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Zhizhong Z. Pant der Problematik, die bedingungslose Gewinnerzielung, wird offensichtlich von Milton Friedman. in dessen viel zitiertem Aufsatz „The Social Responsibility of Business is to Increase its Profits“ markiert. Danach haben Unternehmen ausschließlich die Verantwortung, sich am Markt zu bewähren und ihren

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Molecular Cloning of Opioid Receptors by cDNA Library Screeningd probe is ideal for screening its homologs, or associated splicing variants or full-length cDNAs. If only a partial protein sequence is on hand, degenerate primers can be designed to screen cDNA libraries with a direct polymerase chain reaction (PCR) or with a hybridization procedure. Alternatively

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Analysis of Opioid-Induced Kinase Activationoid signaling to other signal pathways. Furthermore, protein kinases activated by nonopioid signal pathways play important roles in heterologous regulation of opioid functions. Therefore, opioid researchers often face the challenge of determining changes in the activities of protein kinases in study

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Quantitative Single-Cell RT-PCR for Opioid Receptors and Housekeeping Genesces illustrates this approach. In the case of GPD, RT yields one amplifiable cDNA molecule for every 2–3 mRNA molecules. Sensory neurons maintain GPD mRNA in the nanomolar range, but with considerable variability (1.03±0.61 n.). Competitive PCR can be applied to virtually any message sequence. In ne