Titlebook: Opioid Receptors; Methods and Protocol Santi M. Spampinato Book 2021Latest edition Springer Science+Business Media, LLC, part of Springer N

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Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-Tolerant Micecurs has not been completely elucidated to date. One possible hypothesis involves the internalization of MOR. Here, we describe a simple in vitro protocol to investigate the localization of MOR-1 after repeated morphine administration in the spinal cord of morphine-tolerant mice, using western blotting and immunofluorescence techniques.

Prologue

Book 2021Latest editions detail transcriptional and post-transcriptional analysis, cellular detection of opioid receptors, analysis of signaling events modulated by opioid receptors, model systems to studying opioid receptor-mediated functions, and behavioral effects mediated by opioid receptors. Written in the highly suc

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Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Recects. Here a BRET protocol is described to investigate interactions between the kappa opioid receptor (KOR) and nonvisual arrestins (arrestin-2 and arrestin-3) in HEK-293 cells, both under basal conditions and after exposure to KOR ligands.

B-cell

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The Quest for More Effective Analgesics with Reduced Abuse Liability and Fewer Adverse Effects: Promes, pitfalls, and future perspective of biased agonists at mu (MOR) and kappa (KOR) opioid receptors are discussed. Moreover, methodological insights are provided with regard to the most appropriate experimental settings to be employed aiming at developing novel biased KOR agonists.

cunning

MARS

Evaluating Opioid-Mediated Adenylyl Cyclase Inhibition in Live Cells Using a BRET-Based Assayhe assay is based on bioluminescence resonance energy transfer (BRET) that involves transfection of a biosensor in human embryonic kidney (HEK)-293 cells stably transfected with μ-opioid receptor (μ receptor), enabling real-time measurement of cAMP levels.

FAR

An in Vitro Assay to Study the Role of Opioids in Modulating Immune Cell Adhesione cell integrin-mediated adhesion, cell adhesion assay is a simple, reproducible, and valuable tool capable of unraveling concentration-dependent effects of a test candidate on integrin-mediated cell adhesion.

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Book 2021Latest editionreagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... Authoritative and cutting-edge, .Opioid Receptors: Methods and Protocols, Second Edition .aims to ensure successful results in the further study of this vital field..

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