Titlebook: Oogenesis; Methods and Protocol Ioannis P. Nezis Book 2016 Springer Science+Business Media New York 2016 plants.oogenesis.mosquitos.chicken

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Preparation of Cell Lysate from Mouse Oocytes for Western Blotting Analysis,ons in collecting sufficient numbers of oocytes can hinder the efficiency of the technique. Here we provide a detailed protocol for the accurate preparation of mouse oocyte samples for Western Blotting analysis.

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Monitoring Calcium Oscillations in Fertilized Mouse Eggs,m. The monitoring of these Ca. oscillations is a valuable technique to demonstrate that fertilization has occurred, to study egg activation events elicited downstream of the Ca. signal, as well as to evaluate sperm quality. This chapter describes our protocol to monitor sperm-induced Ca. oscillation

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Live Imaging of Intracellular Dynamics During Meiotic Maturation in Mouse Oocytes,onfocal live imaging to mouse oocytes, in which meiotic maturation can be induced in vitro after the introduction of fluorescent proteins through microinjection, the meiotic dynamics of intracellular structures, such as chromosomes, can be monitored at high resolution. A combination of this method w

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Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence-Activated Cell Sorting,Cs) or adult female germline stem cells (aFGSCs), has provided a robust system to study female germ cell development under multiple experimental conditions, and in many species. Flow cytometry or fluorescence-activated cell sorting (FACS) is an integral part of many isolation and characterization pr

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Ioannis P. NezisIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Preparation of , Follicles for Transmission Electron Microscopy,res. This is essential for understanding a large variety of cellular processes. In this chapter, we describe a detailed protocol for preparing . follicles in order to be used as a specimen for transmission electron microscopy.

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Preparation of Cell Lysate from Mouse Oocytes for Western Blotting Analysis,ons in collecting sufficient numbers of oocytes can hinder the efficiency of the technique. Here we provide a detailed protocol for the accurate preparation of mouse oocyte samples for Western Blotting analysis.

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