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Titlebook: Oogenesis; Methods and Protocol Ioannis P. Nezis Book 2016 Springer Science+Business Media New York 2016 plants.oogenesis.mosquitos.chicken

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Preparation of , Follicles for Transmission Electron Microscopy,res. This is essential for understanding a large variety of cellular processes. In this chapter, we describe a detailed protocol for preparing . follicles in order to be used as a specimen for transmission electron microscopy.
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Quantitative Real-Time PCR Analysis of Gene Transcripts of Mosquito Follicles,archers can use either the relative or absolute (standard curve) method to quantify transcript abundance. Characterizing the expression of genes in mosquito ovaries will require use of the standard curve method of quantification. Here, I describe reagents and equipment necessary to run standard curv
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High-Pressure Freezing Electron Microscopy of Zebrafish Oocytes,ous animals are enormous cells endowed with many, big cellular compartments, which are interconnected through active intracellular transport. The dynamic transport pathways and the big organelles of the oocyte provide the opportunity to study cellular trafficking with outstanding resolution. Hence,
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Cytoskeleton and Cytoskeleton-Bound RNA Visualization in Frog and Insect Oocytes,rious structures and molecules are temporarily or permanently bound to the cytoskeletal elements and their functions rely on cytoskeleton integrity and its timely assembly. Thus the accurate visualization of cytoskeleton is often crucial for studies and analyses of oocyte structure and functions. He
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