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Titlebook: Oligodendrocytes; Methods and Protocol David A. Lyons,Linde Kegel Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2

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Myelin: Methods for Purification and Proteome Analysis,d abnormalities in myelin-related disorders. However, reliable molecular profiles necessitate very pure myelin membranes, in particular when considering the power of highly sensitive “omics”-data acquisition methods. Here, we recapitulate the history and recent applications of myelin purification. W
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Teasing of Ventral Spinal Cord White Matter Fibers for the Analysis of Central Nervous System Nodes mediate saltatory conduction in myelinated nerves are concentrated, is orchestrated by oligodendrocytes, the myelinating cells of the CNS. While transmission electron microscopy remains the gold standard for the study of how the nodal region is organized, this approach is both technically demanding
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Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices,s the properties and pharmacology of ion channels, neurotransmitter receptors, and electrogenic transporters, in almost any cell type. In the brain, in addition to neurons, oligodendrocyte precursor cells (OPCs) that give rise to myelinating oligodendrocytes (OLs) are also excitable. Electrophysiolo
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Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Splped characterize different stages of oligodendrocyte maturation in the absence of neurons. However, oligodendrocyte monocultures do not model the interaction of oligodendrocytes with neurons where they form myelin wraps. To circumvent this problem, coculture systems were developed; oligodendrocytes
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