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Titlebook: Nucleic Acids; John M. Walker Book 1984 Humana Press 1984

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发表于 2025-3-21 19:21:14 | 显示全部楼层 |阅读模式
书目名称Nucleic Acids
编辑John M. Walker
视频video
丛书名称Methods in Molecular Biology
图书封面Titlebook: Nucleic Acids;  John M. Walker Book 1984 Humana Press 1984
描述In recent years there has been a tremendous increase in our understanding of the functioning of the cell at the molecular level. This has been achieved in the main by the invention and development of new methodology, parti- larly in that area generally referred to as "genetic en- neering". While this revolution has been taking place in the field of nucleic acids research, the protein chemist has at the same time developed fresh methodology to keep pace with the requirements of present day molecular bi- ogy. Today‘s molecular biologist can no longer be content with being an expert in one particular area alone. He/she needs to be equally competent in the laboratory at h- dling DNA, RNA, and proteins, moving from one area to another as required by the problem he/she is trying to solve. Although many of the new techniques in molecular biology are relatively easy to master, it is often difficult for a researcher to obtain all the relevant information nec- sary for setting up and successfully applying a new te- nique. Information is of course available in the research l- erature, but this often lacks the depth of description that the new user requires. This requirement for in-depth pr- t
出版日期Book 1984
版次1
doihttps://doi.org/10.1385/0896030644
isbn_softcover978-0-89603-107-4
isbn_ebook978-1-59259-489-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1984
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书目名称Nucleic Acids网络公开度学科排名




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书目名称Nucleic Acids读者反馈学科排名




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Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acls, stored indefinitely at −20°C, have full lactate dehydrogenase activity (.) and DNA polymerase activity (.). The dried cells stored for a few days at room temperature also have apparently undegraded nucleic acids (.).
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Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions,lecules is left intact during electrophoresis. The first method describes electrophoresis in a 2% (w/v) agarose gel in a dilute, neutral phosphate buffer. The second deals with electrophoresis in a linear gradient of polyacrylamide based on the buffer system of Loening (.).
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Messenger RNA Fractionation on Neutral Sucrose Gradients,duction of poly(A). mRNA by affinity chromatography (..) the RNA can be fractionated once or twice on sucrose gradients to produce a subpopulation of mRNA enriched for a particular species. The RNA fractions from the gradient can be assayed by in vitro translation and subsequent immunoprecipitation or bioassay of the products (..–.).
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Nucleic Acids978-1-59259-489-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
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,Preparation of “RNase-Free” DNase by Alkylation,Characterization of RNA molecules by electrophoresis or hybridization frequently requires nucleic acid concentrations over 1 mg/mL. High molecular weight DNA in a mixture of nucleic acids limits the solubility and interferes with electrophoresis. DNase treatment makes the mixture more soluble, even if DNA degradation is only partial.
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Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/668784.jpg
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