Titlebook: Nucleic Acid Detection and Structural Investigations; Methods and Protocol Kira Astakhova,Syeda Atia Bukhari Book 2020 Springer Science+Bus

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1064-3745 tection and Structural Investigations: Methods and Protocols .aims present aims to present methodologies combine cutting-edge innovation with sound theory and practical applications in life sciences..978-1-0716-0140-2978-1-0716-0138-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Paresh Prajapati,Wang-Xia Wang,Peter T. Nelson,Joe E. Springern engineering litigation and include. Students in forensic engineering and risk engineering will find the book’s cross-displinary approach an ideal introduction to the subject..978-1-84996-553-8978-1-84628-097-9

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Kira Astakhova,Syeda Atia BukhariIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidi in diagnostic laboratories. Here we describe how automation of PCR reactions using integrated fluidic circuits (IFCs), an IFC controller MX and a Biomark HD instrument allows for the testing of 48 field samples with swine influenza for up to 48 different subtypes simultaneously in nanoliter volumes.

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5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatcheseotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.

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Universal Library Preparation Protocol for Efficient High-Throughput Sequencing of Double-Stranded Rfour main steps, viz., enzyme treatment, precipitation using lithium chloride, full-length amplification of cDNAs, and tailing adapters for high-throughput sequencing. This protocol will be useful for all double-stranded RNA viruses and for all of the high-throughput sequencing platforms.