| 书目名称 | Nuclear Transfer Protocols |
| 副标题 | Cell Reprogramming a |
| 编辑 | Paul J. Verma,Alan O. Trounson |
| 视频video | |
| 概述 | Includes supplementary material: |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear |
| 出版日期 | Book 2006 |
| 关键词 | Activation; gene expression; gene transfer; genes; reproducible techniques; vertebrates |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-59745-154-3 |
| isbn_softcover | 978-1-61737-468-5 |
| isbn_ebook | 978-1-59745-154-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Humana Press 2006 |
发表于 2025-3-21 18:02:08
