Titlebook: Nuclear Receptors; Methods and Experime Mostafa Z. Badr Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Drug d

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Detection of ADP-Ribosylation of the Androgen Receptor Using the Recombinant Macrodomain AF1521 fropending on the superfamily member, Parp enzymes can mono- or poly-ADP-ribosylate a protein substrate. Parp superfamily members confer regulation to a variety of biological processes that include cell signaling, DNA repair, transcription, and stress responses. Here, we describe biochemical methods fo

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Reconstitution of the Steroid Receptor Heterocomplex, factors. Actually, these receptors are steroid-activated transcription factors. Classical soluble receptors exist as oligomeric complexes with the Hsp90-based chaperone machinery. The steroid receptor field was born and developed along with the molecular chaperone field. Chaperones are not exclusiv

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A Reverse Transfection Method for Screening of Nuclear Receptor Activators, of drug-metabolizing cytochrome P450 enzymes. Here, we describe a protocol for a reverse transfection system to study the activation of human nuclear receptors constitutive androstane receptor and pregnane X receptor. The system provides long-term stability and uniformity of DNA–carrier complexes,

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Analysis of the Transcriptional Activity of Retinoic Acid-Related Orphan Receptors (RORs) and Inhibonists on their activity. One assay measures the effect of an inverse agonist on the transcriptional activation of a luciferase reporter by RORs in a Tet-On cell system. A mammalian two-hybrid assay analyzes the interaction of the ROR ligand binding domain with a coactivator peptide. Two additional

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Analysis of IL-4/STAT6 Signaling in Macrophages,-called alternative (M2) activation of macrophages. Interleukin (IL)-4 and IL-13 are important M2 polarizing cytokines that act through STAT6 by inducing its phosphorylation and promoting transcription of STAT6-responsive genes. Inactivation of STAT6 signaling in macrophages has not been fully explo

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Isolation and Characterization of Adipose Tissue Macrophages, this technique can also be used to isolate ATMs from subcutaneous white adipose tissue and brown adipose tissue from mouse, human subcutaneous fat depot, and also from the fat body of the toad .. We detail the flow-cytometric gating strategy that has been developed to identify ATM population, and w