Titlebook: Novel Methods in Molecular and Cellular Biochemistry of Muscle; Grant N. Pierce,William C. Claycomb Book 1997 Springer Science+Business Me

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anaphylaxis

Differential display: Identifying genes involved in cardiomyocyte proliferation,-specific manner determines the fate of the cell and creates the organism. Analysis of this differential gene expression has become a central aim of many laboratories attempting to understand the mechanisms underlying various biological processes. Currently, we are using a technique called different

Stagger

Panther

Analysis of phospholipid molecular species,ospholipid are separated into the diacyl- alkylacyl- and alkenylacyl- subclasses by HPLC on silicic acid. The molecular species of diacylglycerol are separated by HPLC of underivatised diglycerides on a reverse phase octadecyl-silica column. (Mol Cell Biochem .: 129–136, 1997)

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挡泥板

Fura-2 fluorescent technique for the assessment of Ca2+ homeostasis in cardiomyocytes, and extrusion. Ca.-sensitive fluorescent dyes have been used successfully to estimate intracellular free Ca. ([Ca.].) level and the mechanisms of Ca. movements in living cells. This article is focused on the methodology involving the use of Fura-2/AM or free Fura-2 to measure agonist-induced Ca. mo

AMBI

Spectroscopic determination of sarcoplasmic reticulum Ca2+ uptake and Ca2+ release,es from rabbit skeletal muscle. To date, dual-wavelength spectroscopy of arsenazo III and antipyrylazo III difference absorbance have been the most common spectroscopic methods for the assay of SR Ca. transport. The utility of these methods is the ability to manipulate intraluminal Ca. loading of SR

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In-Situ

A simple method for preparation of cultured cardiac fibroblasts from adult human ventricular tissueracellular matrix, growth factors and cytokines in the myocardium, they play an important role in normal and pathologic performance of the heart. An understanding of their biology requires in depth studies in a stable and reliable system in which the biological responses of cardiac fibroblasts to va

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978-1-4613-7918-8Springer Science+Business Media Dordrecht 1997