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Titlebook: Novel Methods in Molecular and Cellular Biochemistry of Muscle; Grant N. Pierce,William C. Claycomb Book 1997 Springer Science+Business Me

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https://doi.org/10.1007/978-1-4615-6353-2DNA; Lipid; Nucleotide; RNA; biochemistry; gene expression; metabolism
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Analysis of phospholipid molecular species,ospholipid are separated into the diacyl- alkylacyl- and alkenylacyl- subclasses by HPLC on silicic acid. The molecular species of diacylglycerol are separated by HPLC of underivatised diglycerides on a reverse phase octadecyl-silica column. (Mol Cell Biochem .: 129–136, 1997)
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Identification of proteins that interact with a protein of interest: Applications of the yeast two-smid library for interacting fusion proteins, elimination of false positives and deletion analysis of true positives. This procedure is designed to allow investigators to identify proteins and their encoding cDNAs that have a biologically significant interaction with your protein of interest. (Mol Cell Biochem . 67–79, 1997)
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Construction of a human heart cDNA library and identification of cardiovascular based genes (CVBestscale single pass sequencing of cDNA clones to generate expressed sequence tags (ESTs) and differential screening of cDNA libraries. The usefulness of combining such approaches for the discovery of novel disease-related and cardiovascular-based ESTs (CVBest) is discussed. (Mol Cell Biochem .: 81–87, 1997)
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Competitive RT-PCR for studying gene expression in micro biopsies,e human cardiac L-type calcium channel. With the method described the dynamic process of cardiac gene expression can be studied in human endomyocardial biopsies in different patient collectives and even in the individual. (Mol Cell Biochem . 89–95, 1997)
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Use of a hammerhead ribozyme with cationic liposomes to reduce leukocyte type 12-lipoxygenase exprebit expression of the 12-LO mRNA will be presented. These results demonstrate the feasibility of using ribozymes as novel candidates for therapeutic agents to block specific gene expression in vascular cells. (Mol Cell Biochem . 47–57, 1997)
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