Titlebook: Nonviral Vectors for Gene Therapy; Methods and Protocol Mark A. Findeis Book 2001 Humana Press 2001

用树皮

Use of Disulfide Cationic Lipids in Plasmid DNA Delivery,ge. Cationic lipo-somes are the most extensively investigated nonviral vectors. It is generally believed that DNA-liposome complexes enter cells via endocytosis, although other pathways such as membrane fusion may exist (.,.). The barriers involved in the transfection process in vitro generally include the following aspects (.):

Arable

四指套

fiscal

Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery,res cellular uptake of polymerDNA complexes, generally referred to as “polyplexes” (.), which most likely occurs via endocytosis, followed by endosomal escape and transport to the nucleus. The polyplex must dissociate, either in the cytosol or in the nucleus, which may be a critical step in the transfection process.

Permanent

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Book 2001emerging in the field of nonviral gene therapy. The book emphasizes not only specific approaches to gene delivery but, in particular, the best current me- ods to prepare, handle, and characterize gene delivery agents. These topics are of very broad importance since gene therapy evolves from its most

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Synthesis of Polyampholyte Comb-Type Copolymers Consisting of a Poly(L-lysine) Backbone and Hyaluroions, e.g., poly(L-lysine) (PLL), are reported to be conjugated with several ligands for targeted gene delivery (.–.). The physicochemical properties of the DNA complexes have been described as factors that influence transfection activity (.–.).

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