Titlebook: Non-Alcoholic Steatohepatitis; Methods and Protocol Devanand Sarkar Book 2022 The Editor(s) (if applicable) and The Author(s), under exclus

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Hydrodynamic Injection for Developing NASH Model, transfection of exogenous DNA primarily in the liver, serving as a reliable approach of establishing animal models for the study of liver diseases. The nonalcoholic steatohepatitis (NASH) is liver inflammation and damage resulting from an accumulation of fat in the liver. With the rising prevalence

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Isolation and Culture of Mouse Hepatocytes and Kupffer Cells (KCs), macrophages (Kupffer cells) play a pivotal role in inducing inflammation. Cross-talk between hepatocytes and Kupffer cells (KCs) regulate both steatosis and inflammation during the pathogenesis of NASH. Isolated hepatocytes and KC serve as important tools to study mechanistic events during NASH in

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Mouse Bone Marrow Cell Isolation and Macrophage Differentiation,, recruited from the bone marrow to the liver, promote NASH-related inflammation and fibrosis. In addition, adipose tissue macrophages (ATMs) release pro-inflammatory cytokines (PICs) which stimulate adipose tissue lipolysis liberating free fatty acids (FFAs) that can accumulate in the liver as trig

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Isolation of the Stromal Vascular Fraction from Adipose Tissue and Subsequent Differentiation into is correlation with obesity, the study of adipose tissue and adipocytes is an important step in understanding the pathogenesis of this disease. Here, we describe the isolation process of the stromal vascular fraction (SVF) of adipose tissue. The SVF contains the foundational cells that will differen

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Culture of Mouse Liver Ductal Organoids,NASH). Hepatic organoids have significant advantages over traditional primary cell cultures. The hepatic progenitor cells can be induced to form hepatic organoids. The established organoids can be passaged or cryopreserved for future use. The established hepatic organoids can be manipulated to study

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Development of a Scalable Three-Dimensional Culture of Human Induced Pluripotent Stem Cells-Derived well as hepatocellular repopulation tool to treat liver metabolic diseases such as nonalcoholic steatohepatitis (NASH). Here we describe a strategy to obtain fully functional liver organoids from hiPSCs in a scalable manner. Our approach uses a two-step process, with a first step involving the scal