书目名称 | Non-Alcoholic Steatohepatitis |
副标题 | Methods and Protocol |
编辑 | Devanand Sarkar |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .This volume contains a comprehensive collection of laboratory protocols used by researchers to analyze varied aspects of non-alcoholic steatohepatitis (NASH). The chapters in this book cover topics such as methods for histological diagnosis of NASH; the purpose of generating an in vivo NASH model; protocols for isolating hepatocytes and Kupffer cells, bone marrow derived macrophages, and adipocytes; techniques to develop human pluripotent stem cells-derived liver organoids; single-cell and RNA-sequencing; and a description of how to extract exosomes and exosomal miRNAs from mesenchymal stem cells. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Thorough and practical, .Non-Alcoholic Steatohepatitis: Methodsand Protocols. is a valuable resource for new and experiences investigators studying NASH, and serves as an essential reference on NASH for basic and clinical researchers and students. . |
出版日期 | Book 2022 |
关键词 | CRISPR; Kupffer cells; Inflammasome; cell death; NASH |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-0716-2128-8 |
isbn_softcover | 978-1-0716-2130-1 |
isbn_ebook | 978-1-0716-2128-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |