Titlebook: Nitric Oxide Protocols; Aviv Hassid Book 2004Latest edition Humana Press 2004

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Acumen

In Vivo Nitric Oxide Measurements Using a Microcoaxial Electrode,its effectiveness as an NO-specific electrode. In this chapter, we describe our experimental protocol for performing real-time NO measurements that was developed during physiological experiments using the microcoaxial electrode.

勉励

Proteomic Method for Identification of Tyrosine-Nitrated Proteins,mass spectrometry or capillary liquid chromatography tandem electrospray mass spectrometry. Nonspecific crossreactivity of the antibodies is determined using reduction of protein bound 3-nitrotyrosine to 3-amino tyrosine using sodium dithionite.

Handedness

Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Corpora Cavernof two gene transfer techniques: adenoviral gene transfer of eNOS and eNOS gene-modified rat marrow stromal cells (rMSCs) to the penis of aged rats for the potential application of gene therapy for the treatment of ED.

Myocyte

Measurement of Nitric Oxide by Reconversion of Nitrate/Nitrite to NO,ion and quantified with an amperometric Clark-type electrode. This method provides the ability to assess basal-and agonist-stimulated cumulative NO formation in different biological models and is a sensitive alternative to the widely used Griess assay.

SIT

Nitrite and Nitrate Measurements in Human Urine by Capillary Electrophoresis,mic sample injections are the main methods of injecting samples onto capillary columns. The urine sample matrix may widely vary depending on urine concentration, urine pH, and ion intake. This chapter illustrates the measurement of nitrite and nitrate in urine by CE and the comparison between electrokinetic and hydrodynamic sample injections.

AVOW

Nitric Oxide Imaging in Neurons Using Confocal Microscopy,the neuron is detected. In this way, nitric oxide is imaged and its intracellular kinetic change is monitored. Furthermore, image processing and visualization techniques are employed to help analyze the image data.

不妥协

Real-Time Polymerase Chain Reaction to Quantify mRNA for Endothelial Nitric Oxide Synthase,ification of mRNA levels, the method should be attractive because of its high sensitivity and the fact that blood vessels from small species such as genetically altered mice can be studied. Here, we demonstrate that the method can measure mRNA levels in cerebral and coronary arteries from mice, in addition to larger blood vessels.

菊花

Dioxygen-Dependent Metabolism of Nitric Oxide,bitors, and nitrate/nitrite assays to measure, characterize, and identify major NO metabolic pathways and enzymes in bacteria, fungi, plants, mammalian cells, and organelles. The methods may prove to be particularly useful for mechanistic investigations and the development of inhibitors, inducers, and other novel NO-modulating therapeutics.

Omniscient

Gene Transfer of Nitric Oxide Synthase Via the Use of Adeno-Associated Virus Vectors, system (adenovirus-free system) and purified by ultracentrifugation using CsCl gradient. The titer of AAV vectors is determined by dot-blot analysis. We have produced the AAV type 2 vectors encoding endothelial NOS (eNOS) gene and transduced rat aortic segments.